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, confirmed a diminished affinity for tyrosine and the activity of your mutant enzyme was roughly fifteen% of the corresponding wild-form action.To determine the kinetic parameters in the enzyme, saturation curves using the substrate (dopamine) were being done. Incubation of your D?H assay mixture well prepared from brains of WT and each with the ?
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This reactive epoxide intermediate may respond with amino acid residues of the CYP enzyme to form drug-CYP (drug-protein) adducts (Plan 6, pathway C). The formation with the drug-CYP adducts may or may not demonstrate a time-dependent inactivation from the catalytic action of the CYP enzyme, with regards to the covalent binding of the reactive inte